Agrobacterium rhizogenes Influences in Vitro Morphogenesis in Stem Segments of Woody Plants

Wild-type Agrobacterium rhizogenes-mediated gene transfer in plants: Agrobacterium virulence and selection of transformants

Agrobacterium rhizogenes ATCC 15834 wild type strain was transformed with the binary vector pBI121 using the heat shock method. The transformed Agrobacterium was then tested for virulence through tobacco leaf explant transformation. Compared to the non-transformed Agrobacterium, the transformed Agrobacterium showed reduced virulence, producing signifi cantly lower number of hairy roots in tobac...

Changes in stem diameter of herbaceous and woody plants as a measure of internal water balance

Optimisation of methods for Agrobacterium rhizogenes mediated generation of composite plants in Eucalyptus camaldulensis

Background The wide adaptability, fast growth rate, good form, excellent wood and fiber properties make Eucalyptus the most widely planted genus of plantation forest trees. With the availability of Eucalyptus genome sequence information, assignment of function to genes contributing to desired traits require functional validation via overexpression or silencing in the context of the plant enviro...

The Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes

The common soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes are unique genetic pathogens capable of fundamentally redirecting plant metabolism in order to generate macroscopic tissue masses (crown galls and hairy roots, respectively) which support the growth of large populations of Agrobacteria. Central to pathogenesis is the horizontal transfer of a suite of oncogenes from ...

In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834

Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...